rabbit polyclonal anti cd47 antibody  (Proteintech)

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: A Case of High-Grade Carcinoma with Medullary Pattern. H&E x100 (A), High CD47 Expression in Tumor Cells (B) (IHC, DAB x200), High infiltration by CD68+TAMs (C) (IHC, DAB x200).

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: A Case of Grade 2 Invasive Duct Carcinoma, NST. H&E x100(A). Low CD47 expression in tumor cells (B) (IHC, DAB x200). Low infiltration by CD68+TAMs (C) (IHC, DAB x200).

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: Associations between the Expression of CD47, CD68 , and Different Clinicopathologic Parameters.

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: Associations between Combined Expression of CD47 and CD68 , and Different Clinicopathological Parameters.

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: Univariate and Multivariate Survival Analysis of the Disease-Free Survival (DFS) and Overall Survival (OS) in breast Carcinoma.

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Immunohistochemical Expression of CD47 and CD68 in Breast Carcinoma and Their Prognostic Value

doi: 10.31557/APJCP.2024.25.7.2515

Figure Lengend Snippet: Kaplan-Meir Survival Curves for Patients with Breast Carcinoma Stratified by CD47 Expression, CD68 Expression, and Combined CD47/CD68 Expression. No statistically significant association between CD47 and DFS (a; log rank; p= 0.06). Significantly lower overall survival (OS) in patients with high CD47 expression compared to those with low CD47 expression (b; log rank; p = 0.004). Significantly lower DFS (c; log-rank; p= 0.03) and OS (d; log-rank; p=0.002) in patients with high infiltration by CD68 +TAMs compared to patients with low infiltration by CD68 +TAMs. Significantly lower DFS (e; log-rank; p≤ 0.001) and OS (f; log-rank; p≤ 0.001) in patients with combined high expression of CD47 and CD68 than in other patients

Article Snippet: Anti- CD47 (Rabbit polyclonal antibody, catalog number A1838, ABclonal, at dilution of 1:100) was used and the immunoreactivity scoring (IRS) system was done using the staining intensity and the percentage of positive cells.

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation

doi: 10.1016/j.jbc.2023.103024

Figure Lengend Snippet: Association of CD47 with Mac-1 on the surface of various Mac-1–expressing cells probed by immunoprecipitation analyses.A, purified WT and CD47-deficient inflammatory peritoneal mouse macrophages were lysed and immunoprecipitated with rabbit polyclonal anti-αM antibody, rabbit polyclonal anti-CD47 antibody, or control rabbit IgG that was used as a specificity control. Blots were analyzed with rabbit polyclonal antibodies against the αM or CD47. The lysates of WT and CD47-deficient macrophages were analyzed by Western blotting using anti-αM and CD47 antibodies. B, murine IC-21 macrophages were lysed and immunoprecipitated with anti-αM rabbit polyclonal antibody or rabbit polyclonal anti-CD47 antibody, and blots were analyzed with rabbit polyclonal antibody against the αM or CD47. C, biotinylated Mac-1-HEK293 cells were lysed and immunoprecipitated with mAb 44a against the αM subunit or isotype control IgG1. Blots were disclosed with streptavidin-conjugated horseradish peroxidase (HRP). The molecular weight of the αM (165 kDa) and β2 (95 kDa) integrin subunits and CD47 (47 kDa) are indicated on the right of the panel. D, suspended (denoted “s”) or adherent (denoted “a”) Mac-1-HEK293 cells were lysed and immunoprecipitated with anti-αM mAb 44a or anti-β2 mAb IB4. Blots were analyzed with anti-αM, anti-β2, and anti-CD47 antibodies. E, the ratios of CD47 to the αM and β2 integrin subunits in the immunoprecipitates from suspended and adherent cells were determined from the densitometry analyses of blots. The ratio of CD47 to each integrin subunit in suspended cells was taken as 1.0. F, lysates of biotinylated Mac-1-HEK293 cells were immunoprecipitated with anti-CD47 mAb B6H12; then immunoprecipitates were subjected to Western blotting probed with streptavidin-HRP (left panel; 1 IP). After the first round of immunoprecipitation, the supernatant was immunoprecipitated with anti-αM mAb 44a (middle panel; 2 IP). The third round of immunoprecipitation (3 IP) was performed using anti-β1 mAb (right panel). M, molecular weight markers.

Article Snippet: The rabbit polyclonal anti-human/mouse CD47 antibody (catalog #CD47-101AP) was from FabGennix.

Techniques: Expressing, Immunoprecipitation, Purification, Western Blot, Molecular Weight

Journal: Frontiers in Cell and Developmental Biology

Article Title: Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis

doi: 10.3389/fcell.2021.745120

Figure Lengend Snippet: Expression of ARID1A and CD47 and their relationship in GC tissues. (A,B) Representative IHC images of ARID1A staining, ARID1A-preserved expression (A) , ARID1A-loss expression (B) . (C,D) Representative IHC images of CD47 staining, CD47 high expression (C) , CD47 low expression (D) . (E) Relationship between loss/preserved expression of ARID1A and CD47 expression ( n = 154). (F) Relationship between mutated/wild-type expression of ARD1A and CD47 expression (data from TCGA, n = 375). * p < 0.05, *** p < 0.001.

Article Snippet: Anti-CD47 rabbit polyclonal antibody (20305-1-AP; 1:50 dilution; Proteintech Group, United States) was applied to identify CD47 expression level in tissues.

Techniques: Expressing, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis

doi: 10.3389/fcell.2021.745120

Figure Lengend Snippet: The correlation between ARID1A status and CD47 expression and clinicopathological characteristics.

Article Snippet: Anti-CD47 rabbit polyclonal antibody (20305-1-AP; 1:50 dilution; Proteintech Group, United States) was applied to identify CD47 expression level in tissues.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis

doi: 10.3389/fcell.2021.745120

Figure Lengend Snippet: Loss of ARID1A increases CD47 expression. (A,B) Protein and mRNA expression levels of ARID1A and CD47 among various GC cell lines. (C–E) Knockdown of ARID1A significantly increased total and membranous CD47 protein levels, and CD47 mRNA level measured by western blot, flow cytometry and qRT-PCR in GC cell lines. (F) Schematic illustration of the relative positions of qPCR probes to putative CD47 promoter region for ChIP-qPCR experiments. (G) ARID1A binding to the promoter region of CD47 was determined by ChIP-qPCR in BGC-823 and MKN28 cells, respectively. *** p < 0.001, **** p < 0.0001.

Article Snippet: Anti-CD47 rabbit polyclonal antibody (20305-1-AP; 1:50 dilution; Proteintech Group, United States) was applied to identify CD47 expression level in tissues.

Techniques: Expressing, Knockdown, Western Blot, Flow Cytometry, Quantitative RT-PCR, ChIP-qPCR, Binding Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis

doi: 10.3389/fcell.2021.745120

Figure Lengend Snippet: Kaplan–Meier curves under various conditions. (A,B) Survival curves according to ARID1A status or CD47 expression ( n = 154). (C) Survival curves according to ARID1A status combined with CD47 expression level ( n = 154). (D–G) Survival curves according to ARID1A status or CD47 expression with HER2 positive or negative.

Article Snippet: Anti-CD47 rabbit polyclonal antibody (20305-1-AP; 1:50 dilution; Proteintech Group, United States) was applied to identify CD47 expression level in tissues.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis

doi: 10.3389/fcell.2021.745120

Figure Lengend Snippet: Characterization of tumor-infiltrating immune cells based on ARID1A and CD47 expression. (A) Estimation of tumor-infiltrating immune cells in different subgroups stratified by ARID1A status and CD47 expression. (B) Multiplex immunofluorescent images illustrate GC tissues with ARID1A loss CD47 high or ARID1A preserved CD47 low expression that express CD11c, CD163, and Foxp3. (C) Quantitative analysis of the densities of immune markers between ARID1A loss CD47 high and ARID1A preserved CD47 low GC tissues. Error bars represent mean with SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Anti-CD47 rabbit polyclonal antibody (20305-1-AP; 1:50 dilution; Proteintech Group, United States) was applied to identify CD47 expression level in tissues.

Techniques: Expressing, Multiplex Assay